Updated
MONITORING ILLUMINA SEQUENCING DATA, TRANSFERRING THEM FROM BASESPACE TO RCC AND TO 10X GENOMICS PLATFORMS
- When:
- Wednesday, May 31, 2023 12:00 pm - 12:50 pm
- Where:
- https://uchicago.Zoom Link: zoom.us/j/5504065119?pwd=Y3I2M0xPME1jTG9yTlcyOHhscUJJdz09#success
- Speaker:
- Jonathan Matthews Tay Lab, SCIC User; MEng, Electrical Engineering and Computer Science (BioEECS), MIT, 2016; BS, Electrical Eng. & Computer Sc., MIT, 2015
- Description:
-
Join us for an informative seminar on the efficient handling of sequencing data in the RCC's flagship HPC clusters, Midway2 and Midway3, housed in the University of Chicago's data center. Our community benefits from state-of-the-art computing infrastructure that provides ample computing power and fast internet speed for enabling discovery and innovation.
The seminar will cover the following topics:
Monitoring sequencing runs in BaseSpace using examples of good and bad quality check scores.
Transferring BaseSpace Sequence Hub data, including Binary Base Call (BCL) and FASTQ files, to the RCC through Midway2 or Midway3 using the command line interface (CLI).
Requeuing BCL files using the BaseSpace BCL Convert App.
Uploading sequencing data from the RCC to the 10X Genomics Platform for processing using the Cell Ranger App.
Join us for this informative seminar to learn how to efficiently handle sequencing data in order to generate meaningful knowledge for scientific purposes.